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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Differences in neurotropism and neurotoxicity among retrograde viral tracers

Fig. 4

Co-injection of rAAV2-retro-Cre-tagBFP and RV-∆G-EGFP into LHA and MPO nuclei to trace input neurons. (a) Schematic diagram of virus co-injection into the LHA of Ai9 reporter mice and the injection sites confirmation. (b-e”) When the LHA was simultaneously injected with rAAV2-retro-Cre-tagBFP and RV-∆G-EGFP, discrepancies in the input circuits retrogradely labeled by the two viruses were observed. The mPFC (Cg1+PrL), M2, PVA, and DI were exclusively labeled by rAAV2-retro-Cre-tagBFP, while the NAc and PVN were preferentially labeled by RV-∆G-EGFP, although overlapping labeling was observed in the MPA. (f) Schematic diagram and the injection sites of virus co-injection in the medial preoptic nucleus (MPO). (g-j”) Injection of rAAV2-retro-Cre-tagBFP in MPO was associated with preferential labeling in the mPFC, AI and PMV. In contrast, injection of RV-∆G-EGFP in MPO was associated with preferential labeling in the LSI. Overlapping signals were observed in the AHiPM. Red, tdTomato expressed by rAAV2-retro-Cre-tagBFP-labeled cells; green, GFP expressed by RV-∆G-EGFP-labeled cells; blue, DAPI. Scale bars = 1 mm for A-D and F-I; 200 μm for B’-D’, G’-I’, B”-D”and G”-I”; 100 μm for E-E” and J-J”

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