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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Differences in neurotropism and neurotoxicity among retrograde viral tracers

Fig. 6

Complementing TVA receptor expression in the mPFC neurons enables EnvA pseudotyped RV infection. (a) Schematic diagram depicting the co-injection of rAAV2-retro-YFP and RV-∆G-DsRed into the LHA. (b-f) Co-injection of rAAV2-retro-YFP and RV-∆G-DsRed into the LHA labeled different input nuclei. The mPFC was labeled by rAAV2-retro-YFP and NAc was labeled by RV-∆G-DsRed. (g) The injection site for rAAV2-retro-YFP and RV-∆G-DsRed in LHA. (h) Schematic diagram depicting the injection of helper AAV expressing TVA (rAAV9-EGFP-TVA) to enable EnvA pseudotyped RV (EnvA-RV-∆G-DsRed) infection in the mPFC. (i-m) Complementation of TVA receptor expression in mPFC neurons enabled EnvA-RV-∆G-DsRed infection in the mPFC. No positive labeling of EnvA-RV-∆G-DsRed was detected in NAc. (n) The injection site for EnvA-RV-∆G-DsRed in LHA. (o) Schematic diagram depicting the injection of EnvA-RV-∆G-DsRed into the LHA without helper rAAV9-EGFP-TVA. (p-t) No signals were observed in the mPFC following injection of EnvA-RV-∆G-DsRed alone. (u) The injection site for EnvA-RV-∆G-DsRed in LHA. Scale bars =1 mm for B, I, P; 200 μm for magnification images

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