Fig. 1
(G4C2)149–mice exhibit hyperactivity, motor deficits, and memory loss at 6 months of age. a Significant increase in distance traveled in (G4C2)149 mice in open field analysis indicative of hyperactivity. b Hanging wire test revealed an increase in the number of falls in (G4C2)149 mice relative to age-matched (G4C2)2 mice. c Cognitive function was assessed by contextual fear conditioning, which demonstrated a significant reduction in freezing in (G4C2)149 mice, indicative of cognitive impairment. d Quantitative analysis of the number of NeuN-positive neurons per mm2 of cortex reveals neuronal loss in (G4C2)149 mice at 6 months of age, but no difference with (G4C2)2 mice at 3 months. e The percent of GFAP immunoreactivity in the cortex demonstrates a significant increase in gliosis in (G4C2)149 mice at both 3 and 6 months relative to control (G4C2)2 mice. Data represent the mean ± SEM. *p < 0.05, **p < 0.01,***p < 0.001, and ****p < 0.0001 as analyzed by unpaired two-tailed t tests (a-c) or 2-way ANOVA (d-e) followed by Tukey’s post-hoc analysis