Fig. 2
Sense and antisense RNA foci observed in the CNS of (G4C2)149–mice. a) Sense RNA foci (white arrowheads) were detected throughout the CNS with representative images from the motor cortex of 3, 6, and 12 month (G4C2)149–mice (bottom panel, a), but not in control (G4C2)2–mice (top panel, a). b-d Percentage of cells containing sense RNA foci in the motor cortex (b), CA1 through CA3 region of the hippocampus (c), and Purkinje layer of the cerebellum (d) in (G4C2)149 mice at 3, 6, and 12 months of age. e) Antisense RNA foci (white arrowheads) were detected throughout the CNS in (G4C2)149–mice (bottom panel, e) but not in control (G4C2)2–mice (top panel, e) at 3, 6, and 12 months of age, with representative images from the motor cortex. f-h) Percentage of cells containing antisense RNA foci in the motor cortex (f), CA1 through CA3 region of the hippocampus (g), and Purkinje layer of the cerebellum (h) in 3, 6, and 12-month (G4C2)149–mice. Data represent the mean ± SEM. *P < 0.05 and ***P < 0.001, as analyzed by one-way ANOVA followed by Tukey’s post-hoc analysis. Scale bar represents 5 μm