Fig. 3

Sense and antisense DPR protein inclusions in (G₄C₂)₁₄₉–mice. a-e Representative images of immunohistochemical analysis of poly(GA), poly(GP), poly(GR), poly(PA), and poly(PR) proteins in the motor cortex of (G₄C₂)₁₄₉ mice at 3, 6, and 12 months of age. f-h Quantitative analysis of the number of poly(GA) (f), poly(GP) (g), or poly(GR) (h) inclusions observed per mm² of motor cortex in (G₄C₂)₁₄₉–mice at 3, 6, and 12-months. i-j Quantitative analysis of poly(PA) (i) or poly(PR) (j) inclusions observed in the cortex of 3, 6, and 12-month (G₄C₂)₁₄₉–mice. Data represent the mean ± SEM. *p < 0.05, **p < 0.01,***p < 0.001, and ****p < 0.0001 as analyzed by 1-way ANOVA followed by Tukey’s post-hoc analysis. Scale bar represents 20 μm