Fig. 5
Deposition of stress granule-associated proteins in (G4C2)149–mice. a-c Representative images of immunohistochemical analysis of G3BP1 (a), eIF3η (b), and ataxin-2 (c) in the cortex of (G4C2)2 and (G4C2)149 mice at 12 months of age, with inclusions indicated by black arrowheads. d Representative images of ataxin-2-positive inclusions in the cortex and hippocampus of (G4C2)149 mice at either 3 or 12 months of age. e-f Quantitative analysis of the number of ataxin-2 inclusions per mm2 of cortex (e) or hippocampus (f) in (G4C2)149 mice. Data represent the mean ± SEM. ****p < 0.0001 as analyzed by unpaired two-tailed t test. Scale bar represents 20 μm