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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice

Fig. 1

Generation and characterization of NOD.Cg-Prkdcscid Il2rgtm1Sug Tg (CMV-IL34)1/Jic (NOG-hIL-34) mice. a NOG-hIL-34 transgenic mice were created in NOD.Cg-Prkdcscidil2gtmlSug/Jic mice by inserting vector containing transgene (Tg), hIL-34, under CMV promoter. b NOG-hIL-34 mice were identified by PCR analysis of ear DNA that amplify hIL-34 (358 bp) in homozygous mice. No bands were detected in non-transgenic NOG controls. A representative gel is shown here. Analysis was done for all 17 NOG-hIL-34 being used in the study and confirmed with the presence of hIL-34 genomic DNA. c hIL-34 expression in plasma was confirmed by ELISA (NOG-hIL-34, n = 6; NOG control, n = 5). d Tissue specific expression of hIL-34 was observed by real time PCR using total RNA isolated from brain, spleen, lung, kidney, liver and skin of NOG-hIL-34 mice (n = 17, except for skin tissue n = 5) compared to NOG controls (n = 5). e, f Establishment of human peripheral hematolymphoid system in CD34-NOG-hIL-34 mice. e Flow cytometry analysis of peripheral blood at 6 months age and gating strategy Representative plots of human cluster of differentiation (CD) 45 positive cells and human CD3, CD19 and CD14 positive cells from human CD45+ gate. f Percentage of human cell subtypes in the peripheral blood of CD34-NOG-hIL-34 mice used in the study. Each symbol represents an individual mouse, n = 17

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