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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Differential effects of diet- and genetically-induced brain insulin resistance on amyloid pathology in a mouse model of Alzheimer’s disease

Fig. 3

Chronic HFD feeding affects the clearance of ISF Aβ. a Secreted Aβ40 and Aβ42 from acute brain slices of 10-month-old A7-Tg mice fed with chow (n = 8) or HFD (n = 9). b Immunoblot and densitometric analyses of IDE protein in the neocortical and hippocampal lysates of 9-month-old A7-Tg mice fed with chow (n = 12) or HFD (n = 11). c NEP activities in microsomal fractions from the cerebral neocortices of 9-month-old A7-Tg mice fed with chow (n = 6) or HFD (n = 5). d-k The levels and half-life of ISF Aβ42 were analyzed by microdialysis. d, h Hourly measurements of ISF Aβ42 during microdialysis in 5 (d) and 9 (h)-month-old A7-Tg mice fed with chow (d: n = 8; h: n = 9) or HFD (d: n = 10; h: n = 11). ISF Aβ42 levels are shown as % of mean baseline of chow-fed mice. e, i Baseline levels of ISF Aβ42 during the 6-h sampling period before compound E treatment in 5 (e) and 9 (i)-month-old A7-Tg mice fed with chow (e: n = 13; i: n = 9) or HFD (e: n = 10; i: n = 11). f, j Rate of reduction in ISF Aβ42 after administration of compound E in 5 (f) and 9 (j)-month-old A7-Tg mice. A semi-log plot of percentage ISF Aβ42 levels (2.0 at time 0) is shown versus time. g, k Half-life of ISF Aβ42 in 5 (g) and 9 (k)-month-old A7-Tg mice fed with chow (g: n = 8; k: n = 9) or HFD (g: n = 10; k: n = 11) based on data in f or j. Data are mean ± SEM. ** p < 0.01 (unpaired t test, a-c, e, g, i, k; repeated-measures ANOVA with Sidak’s post-hoc test, d, h)

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