Fig. 4

Dietary interventions ameliorate HFD-induced metabolic impairments and Aβ pathology in A7-Tg mice. a Experimental scheme. Male A7-Tg mice were fed with HFD for 6 months, followed either by the chow diet (HFD-Chow), 30% calorie-restricted diet (HFD-CR) or remained on HFD for 9 months. b Monthly body weight changes of A7-Tg mice fed with HFD-Chow, HFD-CR or HFD (n = 12 per group). c Fasting plasma insulin levels of 15-month-old A7-Tg mice fed with HFD (n = 10), HFD-Chow (n = 11) or HFD-CR (n = 11). d Fasting blood glucose levels of 15-month-old A7-Tg mice fed with HFD (n = 8), HFD-Chow (n = 10) or HFD-CR (n = 11). e Blood glucose levels of 15-month-old A7-Tg mice fed with HFD (n = 8), HFD-Chow (n = 9) or HFD-CR (n = 11) during the ITT (left) and the AUC of blood glucose (right). f Immunoblot (left) and densitometric (right) analyses of phosphorylated/total IR levels in the cortices of 15-month-old A7-Tg mice fed with HFD (n = 6 and 4), HFD-Chow (n = 6 and 5) or HFD-CR (n = 5 and 6) treated with PBS or insulin. g-h Immunohistochemistry of Aβ deposition (left) and morphometry of percentage Aβ-positive areas (right) in the cerebral neocortices of 15 (f, HFD: n = 10; HFD-Chow: n = 10; HFD-CR: n = 11; Chow: n = 10; CR: n = 11) and 18 (g, HFD: n = 7; HFD-Chow: n = 12; HFD-CR: n = 12; Chow: n = 9; CR: n = 10)-month-old A7-Tg mice. Data are mean ± SEM. *p < 0.05, ** p < 0.01, *** p < 0.001 (repeated-measures ANOVA with Sidak’s post-hoc test, b, e; one-way ANOVA with Dunnett’s post-hoc test, c, d, e; two-way ANOVA with Tukey’s post-hoc test, f; one-way ANOVA with Tukey’s post-hoc test, g, h)