Fig. 6

HFD feeding on IRS-2-deficient A7-Tg mice exacerbates diabetic phenotype and Aβ pathology. a Effect of HFD feeding on body weight of male Irs2^−/−;A7-Tg mice (Chow: n = 5; HFD: n = 6). b Effect of HFD feeding on blood glucose levels of male Irs2^−/−;A7-Tg mice (Chow: n = 5; HFD: n = 6). c Effect of HFD feeding on fasting plasma insulin levels of 9-month-old male Irs2^−/−;A7-Tg mice (Chow: n = 5; HFD: n = 6). d Blood glucose levels during the ITT (left) and the AUC of blood glucose (right) at 9 months of age (Chow: n = 5; HFD: n = 3). e Quantitative RT-PCR analysis of TNFα, CHOP and Grp78/BiP mRNA expression in the adipose tissues of 15-month-old HFD-fed female Irs2^−/−;A7-Tg mice (n = 6 per group). f Soluble (left) and insoluble (right) Aβ40 and Aβ42 levels in the cerebral cortices of HFD-fed male Irs2^−/−;A7 mice at 10 months of age (Chow: n = 6; HFD: n = 5). g Immunohistochemical analysis (left) and morphometry of percentage Aβ-positive areas (right) of the cerebral neocortices of HFD-fed female Irs2^−/−;A7 mice at 15 months of age (n = 6 per group). Data are mean ± SEM. *p < 0.05, **p < 0.01, *** p < 0.001 (repeated-measures ANOVA with Sidak’s post-hoc test, a, b, d; unpaired t test, c-g)