Fig. 1

Neurodegeneration in PINK1 mutant monkey brain. Electron microscopy revealed degenerated cells in the cortex and substantia nigra of a 3-year-old monkey (M6) in which the PINK1 gene was targeted by CRISPR/Cas9 at one-cell embryo stage. Degenerated neurons show electron-dense cytoplasm with no clear organelles and no identifiable nuclear membrane, which are more obvious in dark neurons in the substantia nigra. The controls are the brain regions in a 3-year old male wild type (WT) monkey. The enlarged micrographs beneath the corresponding WT and M6 images are from their boxed areas and show that mitochondria morphology is indistinguishable between WT and M6 neurons. Scale bars: 2 μm. The figures are from authors’ publication [7]