Fig. 10

ICV injection of mimics of miRNAs enriched in NSC-exo reduces hippocampal synaptic vulnerability to Aβo binding. a) Relative levels of expression of miR-322, miR-17 and miR-485 in NSC-exo as compared to MN-exo as determined by small RNA deep sequencing. N = 3 independent NSC-exo or MN-exo preparations each assayed in 3 independent deep sequencing analyses. * p < 0.05 Student’s T-test. b) Mimics of miRNAs (alone or in combination) (1 nmole if injected alone or 0.33 nmoles each if injected in combination) or scrambled miRNA as control were injected ICV 24 h before sacrifice. Hippocampus synaptosomes were prepared and challenged with preformed Aβo (200 nM). After washing, the amount of Aβ in the synaptosomes was quantified by ELISA. *p < 0.05; **P < 0.01 vs. scrambled miRNA. N = 3–4 mice/group. *p < 0.05; p < 0.01 ANOVA (F = 4.796) followed by unpaired T-test multiple comparison analysis.