Fig. 4

The cGMP analog, 8-Br-cGMP, protects against oTau-induced LTP impairment. a Slice perfusion with 8-Br-cGMP (1 μM, 10 min, n = 13) rescued oTau-induced LTP impairment (100 nM, 20 min, n = 8; ANOVA for repeated measures: F_(1,19) = 18.537, p < 0.0001). 8-Br-cGMP alone did not modify potentiation (n = 13 vs. 7 in vehicle-treated slices; F_(1,18) = 0.001, p = 0.974 compared to vehicle). No difference was found between tetanized slices treated with 8-Br-cGMP vs. 8-Br-cGMP + oTau (F_{(1, 24)} = 0.065, p = 0.802). b Residual potentiation at 30 and 120 min after tetanus from data shown in A. (Vehicle: n = 8 slices/7 animals, 3 males and 4 females; oTau: n = 7 slices/7 animals, 3 males and 4 females; 8-Br-cGMP: 13 slices/11 animals, 6 males and 5 females; 8-Br-cGMP + oTau: 13 slices/11 animals, 5 males and 6 females). One-way ANOVA: F_(3,37) = 5.472, p = 0.003 at 30 min and F_(3,37) = 6.670, p = 0.001 at 120 min. Bonferroni’s: p = 0.038 and p = 0.011 between oTau and vehicle at 30 and 120 min, respectively; p = 0.003 and p = 0.002 between oTau and 8-Br-cGMP + oTau at 30 and 120 min, respectively