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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Lack of hepatic apoE does not influence early Aβ deposition: observations from a new APOE knock-in model

Fig. 6

ApoE isoforms and sex differentially influence Aβ accumulation in APP/PS1/EKI mice. Cortical tissues from 4-month-old APP/PS1-EKI mice were sequentially homogenized in PBS and 5 M guanidine-HCl buffer. a The amount of PBS-soluble apoE was quantified by ELISA. There was a significant effect of sex (F1,66 = 8.373, p = 0.0052) and apoE isoform (F2,66 = 3.911, p = 0.0248) but no interaction (F2,66 = 0.8546, p = 0.4301). Post hoc analysis comparing apoE isoform within each sex identified a statistically significant increase in apoE levels in female apoE4-expressing compared to apoE3 (p = 0.0293), b The amount of PBS-soluble Aβ40 was quantified by ELISA. There was a significant effect of apoE genotype (F2,66 = 3.204, p = 0.0470), and a trend towards a significant effect of sex (F1,66 = 3.203, p = 0.0781), with no interaction (F2,66 = 0.06043, p = 0.9414). Post hoc analysis comparing apoE isoforms within each sex did not identify statistically significant pair-wise differences. c The amount of PBS-soluble Aβ42 was quantified by ELISA. There was a significant effect of sex (F1, 66 = 4.246, p = 0.0433) and apoE isoform (F2,66 = 4.943, p = 0.0100) without interaction (F2,66 = 0.5411, p = 0.5847). Post hoc analysis comparing apoE isoform within each sex identified a statistically significant increase in Aβ42 levels in male apoE2-expressing mice compared to apoE3 (p = 0.0338). d The amount of guanidine-soluble Aβ40 was quantified by ELISA. There was a significant effect of sex (F1, 66 = 5.240, p = 0.0253) and apoE isoform (F2,66 = 3.953, p = 0.0239) without interaction (F2,66 = 1.211, p = 0.3044). Post hoc analysis comparing apoE isoforms within each sex identified a statistically significant increase of Aβ40 in female apoE4-expressing mice compared to apoE3 (p = 0.0086). e The amount of guanidine-soluble Aβ42 was quantified by ELISA. There was a trend towards a significant effect of sex (F1, 66 = 3.915, p = 0.0520) and a significant effect of apoE isoform (F2,66 = 5.476, p = 0.0063) without interaction (F2,66 = 1.384, p = 0.2578). Post hoc analysis comparing apoE isoform within each sex identified a statistically significant increase in Aβ42 levels in female apoE4-expressing mice compared to apoE3 (p = 0.0030). 2-way ANOVA, Tukey’s post-hoc test, APP/PS1/E2F = 9 males, 10 females; APP/PS1/E3F = 12 males, 12 females; APP/PS1/E4F = 14 males, 15 females. Data plotted as mean ± SEM. * p < 0.05, ** p < 0.01

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