Fig. 8
Hepatocyte-derived apoE does not influence Aβ accumulation in the brain. a, b, c Brain sections from 4-month-old APP/PS1/EKICre mice and littermates were immunostained with an anti-Aβ antibody. Scale bars = 1 mm g The extent of cortical Aβ deposition in male mice was quantified. There was a significant effect of apoE isoform (F2,49 = 10.63, p = 0.0001), but not Cre expression (F1,49 = 2.693, p = 0.1072) with no interaction (F2,49 = 0.1845). (n; E2F: 8 Cre−/−, 12 Cre+/−; E3F: 10 Cre−/−, 12 Cre+/−; E4F: 5 Cre−/−, 8 Cre+/−). h The extent of cortical Aβ deposition in female mice was quantified. There was a significant effect of apoE isoform (F2,41 = 12.00, p < 0.0001), but not Cre expression (F1,41 = 0.2520, p = 0.6183) with no interaction (F2,41 = 0.09684, p = 0.9079). (n; E2F: 5 Cre−/−, 8 Cre+/−; E3F: 11 Cre−/−, 6 Cre+/−; E4F: 10 Cre−/−, 7 Cre+/−). d, e, f Brain sections from the same cohort were stained with X-34 dye. i The cortical fibrillar plaque load in male mice was quantified. There was a significant effect of apoE isoform (F2,50 = 11.34, p < 0.0001), but not Cre expression (F1,50 = 0.3027, p = 0.5846) with no interaction (F2,50 = 1.607, p = 0.2106). (n; E2F: 8 Cre−/−, 12 Cre+/−; E3F: 11 Cre−/−, 12 Cre+/−; E4F: 4 Cre−/−, 9 Cre+/−). j The cortical fibrillary plaque load in female mice was quantified. There was a significant effect of apoE isoform (F2,42 = 10.53, p = 0.0002), but not Cre expression (F1,42 = 0.5502, p = 0.4624) with no interaction (F2,42 = 0.2734, p = 0.7622). (n; E2F: 5 Cre−/−; 8 Cre+/−; E3F: 11 Cre−/−, 6 Cre+/−; E4F: 11 Cre−/−, 7 Cre+/−). Data analyzed by 2-way ANOVA