Fig. 3
From: Targeting tauopathy with engineered tau-degrading intrabodies
Immunofluorescence and fluorescence in situ analysis of anti-tau intrabody expression in aged P301S-tg mice. a Representative images of anti-HA for protein expression of the various anti-tau intrabodies in 9.5-month-old P301S-tg mice. Immunofluorescent detection of HA reveals clear-cut evidence of the non-targeting anti-tau intrabody within the ipsilateral hippocampus, whereas there was little to no immunofluorescence signal with the modified anti-tau intrabodies. Arrowhead indicating the injection site within the dentate gyrus (dg). b Representative images of in situ hybridization in 9.5-month-old P301S-tg mice validated the expression of the mRNA of the various anti-tau intrabodies within the hippocampus ipsilateral to the AAV injection. Scale bar 200 μM