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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Wild-type Cu/Zn-superoxide dismutase is misfolded in cerebrospinal fluid of sporadic amyotrophic lateral sclerosis

Fig. 1

Sulfonylation of wild-type SOD1 in CSF from some neurodegenerative disease cases. CSF samples (4 μg of total proteins) as well as recombinant human SOD1 proteins (0.1 and 0.25 ng) were analyzed by Western blotting with an anti-SOD1 antibody FL-154. The blots on the membranes were first reacted with FL-154 (0.2 μg/mL) in PBS-T with 1% (w/v) skim milk and then probed with anti-rabbit secondary antibody in PBS-T with 0.5% (w/v) skim milk (1:2000 dilution). A major band corresponding to full-length SOD1 is indicated with a filled arrow, while a band with an open arrow shows a truncated form of SOD1

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