Fig. 6

CSF of ALS cases contains the SOD1 species recognized with several misfolded SOD1-specific antibodies. CSF samples (20 μg of total proteins, 100 μL of volume) were analyzed by sandwich ELISA with (a) apoSOD, (b) EDI, (c) UβB, or (d) 24–39 as a capture antibody. For all cases, Pan-SOD1 antibody was used as a detection antibody. As described in the Methods, absorbance values at 490 nm measured in the ELISA were substituted into equations fitted to standard curves (Additional file 3: Figure S2), and apparent amounts of SOD1 were thereby estimated and plotted as SOD1 (ng/20 μg of total CSF proteins). The data on PD and PSP cases with C4F6-reactive SOD1 were shown as black filled circles. Three independent experiments were performed to estimate error bars (standard deviation). The statistical differences were analyzed with Mann-Whitney U-test, and P values are shown in each panel