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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Autophagy protein NRBF2 has reduced expression in Alzheimer’s brains and modulates memory and amyloid-beta homeostasis in mice

Fig. 5

Injection of rAAV-NRBF2-mCherry into dorsal hippocampus rescues memory impairments and reduces Aβ levels in 5XFAD mice. a-b Y-Maze performance and total number of entries recorded 1 day before (− 1), 30 and 60 days post-viral injection. Each group performance was normalized to their initial performance, i.e. -1 dpi data. Results are mean ± SEM of 4–5 months old WT + mCherry (n = 4), WT + NRBF2 (n = 4), 5XFAD + mCherry (n = 4), 5XFAD + NRBF2 (n = 4) mice. The statistical significance was determined using a repeated two-way ANOVA test. c Immunofluorescence analysis of Aβ and mCherry expression in hippocampus of 5XFAD mice injected with mCherry or NRBF2-mCherry viruses. Scale bars, 500 μm. Images shown are representatives of two different experiments. d Quantification of Aβ signal within CA3 and DG area C. Results were normalized over mCherry control and are mean ± SEM of mCherry or NRBF2-mCherry acquired from three separate mice per group. The statistical significance was determined using regular two-way ANOVA test followed by Bonferroni’s post-test. *p < 0.05

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