Fig. 9
The effects of Dlk or combined Dlk/Lzk deletion on JNK signaling and RGC survival after IA injury. Subjects were Dlkfl/fl or Dlkfl/flLzkfl/fl mice. As elsewhere in this paper, p-JUN expression was used as a marker of DLK-JNK activation. Retinal ganglion cells were labeled with RBPMS. a-d. Representative images of p-JUN (a, c) and RBPMS (b, d) immunostained retinas in which Dlk and Lzk was deleted (AAV-Cre-GFP, c-d) and retinas from fellow eyes in which Dlk and Lzk were left intact (AAV-GFP, a-b). Images illustrate the suppression of p-JUN immunoreactivity at day 3 post-injury (c) and improved RGC survival 30 days post-injury (h) with combined Dlk/Lzk deletion. e-h. Bar graphs with quantitative assessments of the effects of Dlk or Dlk/Lzk deletion on p-JUN expression measured at day 3 after injury (e and f, respectively) and also the effects of Dlk or Dlk/Lzk deletion on RGC survival measured 30 days after injury (g and h, respectively). Scale bars: a-d, 25 μm