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Table 4 rAAV tools which can be applied to studies of neurodegenerative proteinopathies in BSCs

From: Organotypic brain slice cultures to model neurodegenerative proteinopathies

rAAV ToolPromoter(s)Capsid(s)Experimental modelReference(s)
Non-Specific global gene expressionhybrid cytomegalovirus enhancer/chicken β-actin (hCBA)2/1, 2/2, 2/6, 2/8, 2/9BSCs[29]
Microglial expressionCD682/6 with three mutations Y731F/Y705F/T492 V (TM6)BSCs[29]
Neuronal expressionCamKII or MAP-22/6, 2/8BSCs[29]
Oligodendrocyte expressionMBP2/8BSCs[29]
Astrocyte expressionGFAP2/8BSCs[29]
e.g. GCaMP6 or REX-GECO1
Synapsin, chicken β-actin (CAG) or cytomegalovirus (CMV)2/1In vivo rodent, BSCs[95, 96]
Voltage-Sensing e.g. Archon1 or VoltronCamKII or CAGAcute BSCs, Primary Hippocampal Neurons[94]
Retrograde TracingCMV or Synapsin2-retro (Mutant r5H6 (insert LADQDYTKTA + V708I + N382D))In vivo rodent[98]
Optogenetics e.g. Channelrhodopsin-2CAG2/1In vivo rodent, Acute BSCs[99]
Chemogenetics e.g. DREADDsSynapsin2/8In vivo rodent, Acute BSCs[97]
e.g. Cre
CMV2/2In vivo rodent[100]
Gene Editing
e.g. CRISPR/Cas9
CMVDJ, 2/9In vivo rodent[92]
Gene Silencing
e.g. shRNA
Modified CMV, H12/1In vivo rodent[93]