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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway

Fig. 1

αSyn is found in mitochondria-enriched fraction of H4 SL1&SL2 cells and induces a decrease in SIRT3 expression. a Representative cropped western blots showing αsyn and SIRT3 in cytosolic and mitochondrial fractions at different time points (0 – 72 h) (b) αsyn oligomers are apparent after 24 h by luciferase assay (RLU: relative luminescence units), n = 5. Quantification of SIRT3 protein level in mitochondria demonstrates significant decrease in SIRT3 at 48 h and 72 h. c Whole cells lysates from H4 SL1&SL2 cells demonstrate decreased SIRT3 expression after transfection with SIRT3 siRNA n = 6 (d) Luciferase activity from αsyn oligonmerization is significantly increased in cells transfected with SIRT3 siRNA (siSIRT3) compared to control siRNA (siCtrl). Error bars represent the mean ± SD. *p < 0.05, **p < 0.01. Note: In (a) αsyn and SIRT3 bands are from different experiments run on different gels. COXIV, GAPDH, and SIRT3 are all from same samples and immunoblot. Loading controls for αsyn blot are not shown. In panel (c) αsyn, SIRT3, and GAPDH are detected on same immunoblot

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