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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway

Fig. 3

Activation of SIRT3 by AICAR attenuates ROS production. a Fluorescence microscopy images of MitoSox-Red and Mitotracker-Green staining in fixed H4 SL1&SL2 cells. MitoSox-Red fluorescence increases when αsyn is overexpressed and AICAR treatment attenuates mtROS. Representative images from 3 experiments. MitoTracker-Green (mitochondria; green); MitoSox-Red (mitochondria; red); merged images (yellow). Scale bar = 10 μm. b Relative intensity of MitoTracker-Green signal between conditions. Quantification of MitoSOX-Red signal intensities (n = 5), and mean intensity of MitoSOX-Red normalized to MitoTracker-Green intensity (c) Representative cropped western blot of HO-1 and GAPDH in whole cells lysates from H4 SL1&SL2 cells. HO-1 level increases at 72 h and is reduced after AICAR-treatment, n = 5. Error bars represent the mean ± SD. *p < 0.05, **p < 0.01

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