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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway

Fig. 6

αSyn expression decreases SIRT3 and alters mitochondrial dynamics in vivo. a αSyn oligomers were quantified via luciferase assay in rat brain homogenates at 4 weeks after stereotaxic injection of AAV8-SL1&SL2 in SN, n = 5. b Representative cropped western blots showing αsyn, SIRT3, DRP1 in cytosol and mitochondria from SN of rats 4 weeks after stereotaxic injection of AAV8-SL1 and AAV8-SL2. (C). c Quantification of αsyn, SIRT3, DRP1, protein levels in cytosol and mitochondria from two separate blots for each of 4–5 rats. All bands were normalized to respective loading controls GAPDH and COXIV. d Representative cropped western blot of αsyn, DRP1, and p-DRP1 (Ser 616) in SN lysate of AAV8-SL1&SL2 injected rat. αSyn expression leads to increased p-DRP1 protein levels in ipsilateral (I) injected SN compared to contralateral (C) uninjected SN, n = 5 rats total. In panel (a) the same samples were run on one blot that was cropped prior to immunoblotting for αsyn, SIRT3, COXIV, DRP1, and GAPDH. Error bars represent the mean ± SD, (n = 4–5 rats). *p < 0.05, **p < 0.01

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