Fig. 3

Pharmacological chaperone lowers Aβ levels and deposition in 3xTg mice. a Radioimmunoprecipitation assay (RIPA)-soluble and formic acid (FA)-extractable Aβ1–40 levels in cortex of 3xTg mice treated with TPT (3xTg/TPT) or controls (3xTg) were measured by sandwich enzyme-linked immunosorbent assay. Values represent mean ± standard error of the mean (*p < 0.05, n = 6). b RIPA-soluble and FA-extractable Aβ1–42 levels in cortex of 3xTg mice treated with TPT (3xTg/TPT) or controls (3xTg) were measured by sandwich enzyme-linked immunosorbent assay. Values represent mean ± standard error of the mean (*p < 0.05, n = 6). c Representative images of brain sections from 3xTg mice treated with TPT (3xTg/TPT) or control (3xTg) immuno-stained with 4G8 antibody to detect Aβ immunoreactivity (scale bar: 500 μm). d Quantification of the area occupied by Aβ immunoreactivity in brains of 3xTg mice treated with TPT or controls (3xTg). Values represent mean ± standard error of the mean (*p < 0.05, n = 4). e Representative Western blots of amyloid precursor protein (APP), sAPPα, sAPPβ, CTFs, BACE-1, ADAM10, APH-1, Nicastrin, Pen-2, PS1 and SorLA in cortex homogenates from 3xTg mice treated with TPT (3xTg/TPT) or controls (3xTg). f Densitometric analyses of the immunoreactivities to the antibodies shown in the previous panel. Values represent mean ± standard error of the mean (*p < 0.05, n = 3)