Fig. 1
Hyperactive p300/CBP Is Associated with Impairment in the Autophagy-Lysosomal Pathway in Tauopathy Brains a Representative immunoblots of p300, CBP, acH3K18, and H3 in lysates of p300F/F/CBPF/F primary neurons infected with lenti-control (ctrl) or lenti-Cre. b Levels of acH3K18, measured by ELISA, in CSF samples from normal controls (normal, n = 14) and patients with AD (n = 13). ***p < 0.001 by unpaired t test. Values are mean ± SEM. c Pearson correlation analysis of acH3K18 and CSF p-tau/Aβ42 levels in CSF samples from normal controls (normal, n = 3) and AD patients (n = 6). d Representative immunoblots of acH3K18, total H3, LC3-I, LC3-II, SQSMT/p62, and GAPDH in hippocampal lysates of 10-month-old wildtype (WT) and PS19 mice. e–g Levels of acH3K18 relative to total H3 (e), LC3-II (f) and p62 (g) relative to GAPDH, normalized to WT. h–j Pearson correlation analysis of LC3-II and p62 levels (h), acH3K18 and LC3-II levels (i), and acH3K18 and p62 levels (j). (d–j) n = 7 mice per group. **p < 0.01, ***p < 0.001 by unpaired t test. Values are mean ± SEM