Fig. 3

Lrrk2-deficiency accelerates nuclear hypertrophy during aging. a Co-staining of CTIP2, Darpp-32 and MOR1 in patch and matrix compartments of 12-month-old Lrrk2^+/+ and Lrrk2^−/− mice. Scale bar, 5 μm. b, c The soma (b) and nucleus (c) size of SPNs in the patch and matrix compartments of 12-month-old Lrrk2^+/+ and Lrrk2^−/− mice. N = 5 mice per genotype. 600–100 neurons were counted per animal. Conditional logistic regression test, ^****p < 0.0001. d Cumulative (Cum.) frequency of the soma and nuclear size distribution in the patch and matrix compartments of Lrrk2^+/+ and Lrrk2^−/− mice. e The soma area of SPNs in the patch and matrix compartments of Lrrk2^+/+ and Lrrk2^−/− mice at 2 (n = 3 mice per genotype, 50–80 neurons per animal), 12 (n = 5 mice per genotype, 60–100 neurons per animal) and 24 months (n = 3 mice per genotype, 60–90 neurons per animal) of age. 2way NOVA analysis with Sidak’s multiple comparisons test, ^*p = 0.025 (patch soma), ^*p = 0.0118 (matrix soma). f The nuclear size of SPNs in the patch and matrix compartments of Lrrk2^+/+ and Lrrk2^−/− mice at P0 (n = 3 mice per genotype, 60–100 neurons per animal), 2 (n = 6 mice per genotype, 50–200 neurons per animal), 12 (n = 8 mice per genotype, 60–150 neurons per animal) and 24 months (n = 3 per genotype, 60–90 neurons per animal) of age. 2way ANOVA analysis with Sidak’s multiple comparisons test, ^***p = 0.0005 (patch nuclei), ^***p = 0.0002 (matrix nuclei). g The nuclear size and soma size ratio (N/C ratio) of SPNs in the patch and matrix compartments of Lrrk2^+/+ and Lrrk2^−/− mice. The number of mice and neurons as indicated in e and f. 1way ANOVA analysis with Sidak’s multiple comparisons test, no statistically significant difference was identified