Fig. 5

Forebrain atrophy and reduced dendritic complexity in the SPNs in 12-month-old Lrrk2^−/− mice. a Co-staining of Darpp-32 and DAPI in the forebrain coronal sections of 12-month-old Lrrk2^+/+ and Lrrk2^−/− mice. The dorsal striatum (DS) and ventral striatum (VS) are highlighted with red and blue colors, respectively. Scale bar, 500 μm. b The volumes of frontal cerebral cortex (FC), DS and VS. N = 5 mice per genotype. Unpaired t-test, ^*p = 0.034 (FC); ^*p = 0.037 (DS); p = 0.856 (VS). c The numbers of SPNs in the DS. N = 5 mice per genotype. Unpaired t-test, p = 0.387 of 12-month-old Lrrk2^−/− mice. d The GFP-labeled SPNs (top panel). 3D reconstruction of the top fluorescent images (bottom panel). Scale bar, 50 μm. e, f Sholl analysis of dendritic complexity of GFP-labeled SPNs. N = 5 mice per genotype. 5–9 neurons were examined per animal. Benjamin-Hochberg multiple comparison test of dendritic complexity at 24, 30, 36, 42, 48, 54, 60, 72, 84 and 132 μm from soma, q ≤ 0.05. g Dendritic length of GFP-labeled SPNs. N = 5 mice per genotype. 5–9 neurons were counted. Unpaired t-test, ^****p < 0.0001. h Soma volume of GFP-labeled SPNs. N = 5 mice per genotype. For each animal, 5–9 neurons were counted. Unpaired t-test, ^**p = 0.0086