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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS

Fig. 4

In vivo transduction via somatic brain transgenesis. Media containing rAAV2-EGFP vectors was injected directly into each lateral ventricle of non-transgenic mice at postnatal day 0 (P0). a Representative brain sections from mice aged P30 stained with an anti-EGFP antibody (n = 3–5). Bar, 500 μm. b Higher magnification (10X) images show transduction by select mutant rAAV2/2, 2/8, and 2/9 capsids. Bar, 150 μm (c) Immuno-fluorescent co-staining for EGFP expression (green) and cell-type specific markers (red) reveals transduction of astrocytes (anti-GFAP) and neurons (anti-MAP2) but not microglia (anti-IBA1) Bar, 50 μm. Additional co-localization data can be found in Fig. S3

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