Table 1 PEI transfection to produce rAAV in 6-well plate
AAV double transfection | AAV triple transfection | PEI solution | |||
|---|---|---|---|---|---|
Transgene plasmid | 675 ng | Transgene plasmid | 675 ng | PEI | 30 μl |
Capsid-helper plasmid | 2025 ng | Capsid-helper plasmid | 675 ng | 1.5 M NaCl | 10 μl |
1.5 M NaCl | 10 μl | Adeno helper plasmid | 1350 ng | Water | 60 μl |
Water | to 100 μl | 1.5 M NaCl | 10 μl | Total | 100 μl |
Total | 100 μl | Water | to 100 μl |  |  |
|  |  | Total | 100 μl |  |  |