Skip to main content

Table 3 PEI Transfection to produce concentrated rAAV preparations

From: Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS

AAV double transfectionAAV triple transfectionPEI solution
Transgene plasmid12.5 μgTransgene plasmid12.5 μgPEI0.5 mL
Capsid-helper plasmid37.5 μgCapsid-helper plasmid25 μg1.5 M NaCl0.1 mL
1.5 M NaCl0.1 mLAdeno helper plasmid12.5 μgWater0.4 mL
Waterto 1 mL1.5 M NaCl0.1 mLTotal1 mL
Total1 mLWaterto 1 mL  
  Total1 mL