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Table 3 PEI Transfection to produce concentrated rAAV preparations

From: Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS

AAV double transfection

AAV triple transfection

PEI solution

Transgene plasmid

12.5 μg

Transgene plasmid

12.5 μg

PEI

0.5 mL

Capsid-helper plasmid

37.5 μg

Capsid-helper plasmid

25 μg

1.5 M NaCl

0.1 mL

1.5 M NaCl

0.1 mL

Adeno helper plasmid

12.5 μg

Water

0.4 mL

Water

to 1 mL

1.5 M NaCl

0.1 mL

Total

1 mL

Total

1 mL

Water

to 1 mL

  
  

Total

1 mL

 Â