Fig. 1

Ubiquitous expression of PARIS in Drosophila results in partial lethality, shortened lifespan and climbing defects. a Immunoblot analysis of PARIS and PARIS mutant (C571A) protein levels in independent transgenic fly lines as indicated, N = 3. b Actin-Gal4 driven ubiquitous expression of PARIS causes partial lethality during development while C571A has no lethal effects. Eclosion rate normalized to control (Act-Gal4/+). c Kaplan Meier survival curve showing substantially reduced life span in Act>PARIS flies compared to Act-Gal4/+ control. Also see Additional file 3, Table S3 (d) Ubiquitous expression of PARIS but not C571A leads to a significant and age-related progressive decline in climbing performance that is restored by L-DOPA treatment. Act-Gal4/+ flies served as control. N = 60–80 flies per group. Quantitative data = mean ± SEM, One-way ANOVA ***p < 0.001, ****p < 0.0001