Fig. 2
From: α-Synucleinopathy associated c-Abl activation causes p53-dependent autophagy impairment
Activated c-Abl and p53 accumulates in neurons exhibiting αS pathology. a, b Pathological αS accumulation in the brainstem of end-stage TgA53T Hu αS mice were detected using anti-pS129αS antibody (Red). The sections were also double immune-stained for activated c-Abl (pY245c-Abl, Green) (a) or activated p53 (pS15p53, Green) (b). Arrows indicate colocalization of active c-Abl (a) and p53 (b) with neurons that accumulate pS129αS. No significant staining for pS129αS, pY245c-Abl, or pS15p53 are seen in nTg mice. Scale, 20 μm. c. Spinal cord (SPC) sections from nTg and end stage TgA53T mice were immunostained using a pan-p53 antibody followed by a confocal fluorescence imaging. Note the overall increase in the p53 immunoreactivity in TgA53T mice compared to nTg mice. Arrows shows the nuclear localization of p53 in both nTg and Tg. Arrowhead shows the cytosolic localization of p53 seen only in the TgA53T mice. Scale, 10 μm