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Fig. 9 | Molecular Neurodegeneration

Fig. 9

From: α-Synucleinopathy associated c-Abl activation causes p53-dependent autophagy impairment

Fig. 9

Modulation of mTOR signaling by nilotinib (Nilo) treatment in vivo. Six-month-old TgA53T mice were subjected to IC/IS injection with 3000×g brainstem/spinal cord lysates from end stage mice as described in “Materials and Methods”. Fourteen days post IC/IS injection, Nilo or DMSO were administered for 45 days (i.p. 3× per week) prior to harvesting of the brain regions. a A brief experimental time line. b Total lysates from the brainstem (BST) of experimental subjects were immunoblotted for pS129αS, αS, LC3, p62, pS6, S6, p4EBP1, 4EBP1 and α-Tub. Quantitative analysis for pS129αS/αS (c), LC3/p62 (d), pS6/p4EBP1 (e) shows that Nilo treatment reduces αS pathology and decreases mTOR activity at presymptomatic stages. All values represent mean ± SEM, n = 3–4, *p < 0.05; **p < 0.01, Student’s t test

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