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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Dipeptide repeat proteins inhibit homology-directed DNA double strand break repair in C9ORF72 ALS/FTD

Fig. 3

DNA repair efficiency in response to the manipulation of nucleophosmin levels. a-b) Repair efficiency (Y-axis) as determined by the percentage of GFP positive cells in cell cultures transfected with proline-arginine (PR), glycine-arginine (GR), glycine-alanine (GA) and nucleophosmin siRNA, or control siRNA. Two reporter cell lines were used to assess the efficiency of (a) non-homologous end joining (NHEJ) and (b) single strand annealing (SSA). Statistical significance was assessed by one-way ANOVA and post-hoc test between each experimental group and the control group (control siRNA); n = 6 biological replicates, 100,000 cells/replicate were evaluated; error bars are SEM, *p < 0.05, ***p < 0.0005, ****p < 0.0001. (c-f) Representative fluorescence activated cell sorting plots of transfected NHEJ and SSA reporter cells using GFP fluorescence (Y-axis) and side scatter (X-axis); the number of GFP positive cells is represented as a percentage of parent gating

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