Fig. 5

Expression of activated and total RAD52 in C9ALS/FTD neurons. a) Quantification of pRAD52 mean fluorescence in neurons using automated image analysis software (Fiji/Image J) for three C9ALS iPSC lines normalized to isogenic lines; each data point represents one cell. b) Representative confocal immunofluorescence images of iPSC motor neuron cultures stained with an antibody against phospho-RAD52 (pRAD52) (green) and counter-stained with DAPI (blue) scale bars are 100 μm. c) Quantification of total RAD52 (tRAD52) mean fluorescence normalized to isogenic line. d) Representative images of iPSC motor neuron cultures immuno-stained with an antibody against RAD52 (tRAD52) (green) and DAPI (blue). n = 3 biological replicates, 5 fields per replicate, error bars are SEM; ****P < 0.0001, as determined by unpaired student’s t test