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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Premature termination codon readthrough upregulates progranulin expression and improves lysosomal function in preclinical models of GRN deficiency

Fig. 6

Selective increase of R493X−/− KI nonsense mutant mRNA by PTC readthrough treatment. qPCR analysis of human GRN mRNA levels in DIV 50 WT and R493X−/− KI hiPSC-derived cortical neurons (a) and DIV60+ astrocytes (b) treated with G418 and CDX5–288 at the indicated concentrations for 72 h. Relative GRN mRNA levels were normalized to the mean of GAPDH and HPRT1 housekeeping genes and compared to vehicle-treated (VT) WT. n = 3 independent cultures; values are shown as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.0001 was determined by one-way ANOVA with Tukey’s multiple comparison test

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Molecular Neurodegeneration

ISSN: 1750-1326