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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: A role for α-Synuclein in axon growth and its implications in corticostriatal glutamatergic plasticity in Parkinson’s disease

Fig. 7

The dopaminergic system in A53T α-Syn tg mouse brains. a Coronal brain sections (6μm, bregma + 1.3- (− 3.8)) of A53T α-Syn tg mouse (14 months), immunoreacted with anti Tyrosine hydroxylase (TH) antibody. CC, corpus callosum; CPu, caudate putamen; LV, lateral ventricle; mfb, medial forebrain bundle; NAc, nucleus accumbens; OT, olfactory tubercle; VP, ventral pallidum; VTA, ventral tegmental area. 3 V, third ventricle. Bar = 500 μm. B-C. Higher magnification of brain sections (as in a). b SNc, Bar = 25 μm. c Striatal WMT. Bar = 20 μm. d Protein samples (30 μg) of striatal homogenates from 12 to 14 month old A53T α-Syn tg and age matched α-Syn −/− control mouse brains analyzed by Western blotting. Blot immunoreacted with antibodies for dopamine transporter (DAT); Tyrosine hydroxylase (TH); β-actin; and synaptophysin (SP). A representative blot out of two. N = 5 brains in each genotype. e A graph showing quantitation of blots in (d), values normalized to β-actin levels in the same sample. Vertical bar represents control brains, set at 100%. f Quantitative values of Western blot as in (d) reacted with anti vGluT1 ab. g Immunohistochemistry (IHC) of paraffin embedded mouse brain section co-immunoreaced with anti vGlut1 (red) and anti NF-200 (green) abs. Bar = 25 μm. h IHC of a paraffin embedded section containing the olfactory tubercle from an A53T α-Syn tg mouse brain at 12 month of age, co-immunoreaced with anti TH ab. i Quantification of immunoreactivity obtained by IHC in the olfactory tubercle. Paraffin-embedded brain sections of A53T α-Syn tg and control 12–14 months old mice, immunoreacted with anti TH antibody as in (h). Mean ± SD of n = 4 mouse brain. *, P < 0.05, ttest. Bar = 100 μm

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