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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer’s disease

Fig. 2

Enrichment of microglia-specific proteins and depletion of non-microglial proteins by FACS. a Scatter plot displaying relative abundance of all differentially expressed proteins (n = 953) between FACS-isolated microglia proteome and MACS-enriched microglia proteome (all dots represent p < 0.05). Dotted lines differentiate proteins with a Log2 fold-change of at least 2. Solid orange dots indicate proteins significantly higher in the FACS proteome or lower in MACS proteome. Solid blue dots indicate proteins significantly higher in MACS proteome or lower in FACS proteome. b Results from Gene Ontology (GO) enrichment analysis of 953 differentially expressed proteins against a background list of total proteins identified in current dataset. Representative GO terms (top 3) from each of the three GO groups. Degree of enrichment of each GO term is indicated by the Z-score (X-axis). Vertical red and black lines indicate a Z-score of 1.96. c Volcano plot displaying the distribution of differentially expressed proteins between FACS-isolated and MACS-enriched microglia proteomes. Cell type-specific markers defined by a reference proteome, Sharma et al. [22], shows significant enrichment of microglial specific proteins in the FACS proteome (p < 0.05, Unpaired t-test). Red dots = microglia, turquoise dots = neuron, pink dots = astrocyte, yellow dots = oligodendrocyte. Grey dots represent differentially expressed proteins with a p > 0.05. Log2 fold-change is shown on the X-axis, −Log10 (p-value) is shown on the Y-axis, and horizontal dotted line indicates p value of 0.05. d Consensus microglial proteins between MACS and FACS microglial proteomes (n = 203). From this list, Moesin (Msn) was chosen for further validation

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