Fig. 2
From: Interleukin-6 deficiency exacerbates Huntington’s disease model phenotypes
IL-6 deficiency exacerbates the R6/2 HD model behavioral phenotype. Experimental groups: WT_WT: R6/2 non-carrier and IL-6+/+; WT_KO: R6/2 non-carrier and IL-6−/−; R6/2_WT: R6/2 hemizygous carrier and IL-6+/+; R6/2_KO: R6/2 hemizygous carrier and IL-6−/−. a. Body weight measurements over time show that R6/2_WT mice lose more weight than the R6/2_KO mice as compared to WT_WT or WT_KO controls. Mixed effects model (restricted maximum likelihood REML), p < 0.0001, Tukey’s multiple comparison p = 0.027 (*), p = 0.0005 (***), p < 0.00001 (****). Number of animals per group: WT_WT (n = 10), WT_KO (n = 5), R6/2_WT (n = 10), R6/2_KO (n = 14). b. R6/2_KO mice perform more poorly than R6/2_WT mice on the rotarod assay at 5–7 weeks of age. Number of animals per group: WT_WT (n = 10), WT_KO (n = 5), R6/2_WT (n = 10), R6/2_KO (n = 13). Mixed effects model (restricted maximum likelihood REML), p < 0.05, Tukey’s multiple comparison p < 0.01 (**), p < 0.0001 (****). c-e. R6/2_KO mice demonstrate less spontaneous motor activity than R6/2_WT mice, as measured by horizontal distance traveled, ambulatory time, and vertical episodes in the open field assay at 7 weeks of age. Number of animals per group; WT_WT (n = 10), WT_KO (n = 5), R6/2_WT (n = 10), R6/2_KO (n = 12). p < 0.01 (**), two-tailed t-test. f-h. R6/2_KO mice demonstrate less spontaneous motor activity than R6/2_WT mice, as measured by horizontal distance traveled, ambulatory time, and vertical episodes in the open field assay at 10 weeks of age. Number of animals per group: WT_WT (n = 10), WT_KO (n = 5), R6/2_WT (n = 9), R6/2_KO (n = 11). p < 0.01 (**), two-tailed t-test. All data are represented as mean ± standard error of the mean