Fig. 2

Human/mouse mismatches at positions 121 and 122 affect aSyn neurotoxicity in cell culture. Primary midbrain cultures were transduced with adenovirus encoding human, mouse, and chimeric aSyn variants at MOIs adjusted to ensure equal expression levels. Additional cultures were untransduced (‘control’). The cells were fixed, stained with antibodies specific for MAP2 and TH, and scored for dopaminergic cell viability. The data are presented as the mean ± SEM, n = 4 (a, b), n = 5 (c), or n = 3 (d-f). *p < 0.05, **p < 0.01, ***p < 0.001; square root transformation, one-way ANOVA followed by Tukey’s multiple comparisons post hoc test. The datasets represented by panels a-d and f are partially overlapping with respect to replicate data obtained for Control, h-asyn A53T, and m-asyn