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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Two C-terminal sequence variations determine differential neurotoxicity between human and mouse α-synuclein

Fig. 5

M-aSyn forms amyloid-like fibrils with a shorter lag time compared to human and chimeric aSyn variants. The formation of amyloid-like fibrils was monitored in solutions of monomeric h-aSyn WT, h-aSyn A53T, and m-aSyn (a), or h-aSyn A53T, h-aSyn Chimera, m-aSyn, and m-aSyn Chimera (b) (35 μM of each). The protein solutions were incubated at 37 °C with constant agitation and analyzed at various times for thioflavin T fluorescence. The graphs show the mean normalized fluorescence (determined from 3 independent experiments; 3 technical replicates per experiment) plotted against the incubation time. The dotted lines above and below each solid line correspond to the mean signal ± SEM. See also Figure S3

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