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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Sex-dependent calcium hyperactivity due to lysosomal-related dysfunction in astrocytes from APOE4 versus APOE3 gene targeted replacement mice

Fig. 2

Equal Ca2+ signals in astrocytes from female APOE3 and APOE4 targeted replacement mice. a Spontaneous Ca2+ activity in astrocytes from APOE3 (N = 26 astrocytes) and APOE4 female mice (N = 35 astrocytes). Ca2+ was monitored for 120 s without any stimulation. b Left panels, raster plots of Ca2+ activity in astrocytes from APOE3 (upper panel, N = 165 astrocytes), and APOE4 female mice (lower panel, N = 124 astrocytes). The color code indicates relative fluorescence changes before and after local application of ATP (arrow, 5 s, 1 mM). Right panel, representative traces of Ca2+ signals evoked by an ATP puff in APOE3 (upper panel) and APOE4 (bottom panel) astrocytes (arrows indicate ATP stimulation). c Quantification of the amplitude and frequency of Ca2+ events for 30 s before (basal peaks), during (ATP) and after local stimulus (post-ATP) in APOE3 and APOE4 astrocytes. Statistical significance was established at p < 0.001 (***); One-way ANOVA followed by Dunn’s post hoc test. All the experiments were performed in the presence of TTX (1 μM). N = 4 mice, for both APOE3 and APOE4

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