Fig. 6

Loss of modulation of Ca²⁺ signals by lipids in APOE4 astrocytes. Representative traces of 100 μM ATP-induced Ca²⁺ responses in APOE3 and APOE4 immortalized cells in (a) saline medium or Krebs medium (KH) for 2 to 5 min, (b) DMEM medium supplemented with lipoprotein-deficient serum (Lipoprotein (−)) for 2 to 5 min, (c) DMEM medium supplemented with B27 for 2 to 5 min, and (d) overnight (ON), and (e) DMEM medium supplemented with 10% FBS for 5 min. f Quantification of the ATP-induced intracellular Ca²⁺ peak in cells kept in the different mediums (N = 3). Two-way ANOVA was used. p < 0.01 (**) as compared to APOE3 cells kept in the same medium