Fig. 3
From: Impaired NHEJ repair in amyotrophic lateral sclerosis is associated with TDP-43 mutations
A315T and Q331K mutants do not prevent DNA damage in primary neurons. Top/middle panels; Confocal microscopy of mouse primary cortical neurons transfected with wildtype TDP-43, mutants A315T or Q331K, EGFP only (EGFP), or untransfected cells (Un), treated with 13.5 μM etoposide for 1 h. Immunocytochemistry was performed using anti-γH2AX antibodies. Scale bar 10 μm. Bottom panel; Quantification revealed less DNA damage γH2AX foci in neurons expressing wildtype TDP-43 after etoposide treatment compared to controls. In contrast, cells expressing ALS-associated mutants A315T and Q331K are not protected from damage compared to wildtype TDP-43. Two-way ANOVA with Tukey correction Mean ± SEM, *p  < 0.05, ***p < 0.001, N = 3, at least 20 cells/group were counted