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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Impaired NHEJ repair in amyotrophic lateral sclerosis is associated with TDP-43 mutations

Fig. 4

Wildtype TDP-43 prevents DSB accumulation in contrast to ALS-linked mutants A315T and Q331K. Neutral comet assay of Neuro2A cells expressing wildtype, Q331K, A315T or Empty vector (EGFP), or untransfected cells, only, treated with 13.5 μM etoposide for 1 h. a Representative images of comets. b Quantification of comets expressed as the Olive Tail Moment (OTM) revealed less DNA DSBs in cells expressing wildtype TDP-43 compared to Q331K, A315T mutants or EGFP and untransfected cells. Two-way ANOVA with Tukey correction Mean ± SEM, ****p < 0.0001, ** p < 0.01, * p < 0.05. N = 3, at least 50 cells/group were analysed

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