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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filaments

Fig. 3

Full length wildtype Tau proteins (with or without GFP) induce microtubule assembly whereas the repeat domain does not. (a) Assembly of microtubules (10 μM tubulin) is supported by TauFL-wt (5 μM) without GFP or with GFP attached to either N- or C-terminus (top, green, blue, magenta curves). MT assembly levels and rates (t1/2 ~ min range) are comparable. Note that GFP alone shows no increase in light scattering (red curve). (b) Microtubule assembly is not supported by the repeat domain TauRDΔK, regardless of GFP (bottom curves: TauRDΔK (light green); GFP-TauRDΔK (blue); TauRDΔK–GFP (magenta); GFP alone (red)). As a control, TauFL-wt induces robust microtubule assembly (as in a, green). Note that the repeat domain R1-R4 is often denoted as “MTBD” domain in the literature, even though it binds and assembles microtubules poorly

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