Fig. 6From: FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filamentsSTEM imaging reveals that packing of Tau in assembly conditions is severely altered by GFP tags. (a) Mass per length histograms (MPL) of fibrils aggregated for 24 h at 37 °C, with fitted peak values (in kDa/nm) listed for each protein. Examples of STEM images are shown for TauFL-wt(b), GFP-TauFL-wt(c), TauRDΔK(d); TauRDΔK-GFP fibrils (long) (e) and TauRDΔK-GFP fibrils (short) (f). MPL measurements were calibrated with tobacco mosaic virus (TMV, shown in the bottom histogram in a and in image g). The estimated molecules per nm are presented in Table 1. Note that TauRDΔK-GFP fibrils have been observed mostly as short and stubby fibrils, but that the long fibrils show a packing, less dense than the short fibrilsBack to article page