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Table 1 Summary of the effects of Tau + GFP fusion proteins on aggregation, stimulation of microtubule assembly, and packing of subunits in Tau filaments

From: FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filaments

Construct

Expression (Cell type)

MT assembly

Aggregation (+Heparin)

Mol. weight (kDa)

MPL (kDa/nm)

No.of. molecules /nm

TauFL-wt

E.coli

yes

Fibrils

45.85

157.1±4

3.4

GFP-TauFL-wt

E.coli

yes

Fibrils

73.76

163.0±5

2.2

TauFL-wt-GFP

E.coli

yes

Fibrils

74.54

n.d.

TauRDΔK

E.coli

no

Fibrils

13.55

61.5±4

4.5

GFP-TauRDΔK

E.coli

no

no

43.07

n.a.

TauRDΔK-GFP

Sf9 cells

no

Short fibrils (minor fraction)

42.77

86.1±5

2.0

Long fibrils (minor fraction)

42.77

37.4±7

0.87

TMV

131.1±1 kDa (standard)