From: FRET-based Tau seeding assay does not represent prion-like templated assembly of Tau filaments
Construct | Expression (Cell type) | MT assembly | Aggregation (+Heparin) | Mol. weight (kDa) | MPL (kDa/nm) | No.of. molecules /nm |
---|---|---|---|---|---|---|
TauFL-wt | E.coli | yes | Fibrils | 45.85 | 157.1±4 | 3.4 |
GFP-TauFL-wt | E.coli | yes | Fibrils | 73.76 | 163.0±5 | 2.2 |
TauFL-wt-GFP | E.coli | yes | Fibrils | 74.54 | n.d. | |
TauRDΔK | E.coli | no | Fibrils | 13.55 | 61.5±4 | 4.5 |
GFP-TauRDΔK | E.coli | no | no | 43.07 | n.a. | |
TauRDΔK-GFP | Sf9 cells | no | Short fibrils (minor fraction) | 42.77 | 86.1±5 | 2.0 |
Long fibrils (minor fraction) | 42.77 | 37.4±7 | 0.87 | |||
TMV | 131.1±1 kDa (standard) |