Fig. 3
From: Trem2 deficiency differentially affects phenotype and transcriptome of human APOE3 and APOE4 mice
Trem2 deletion affects microglia barrier and plaque growth at 4.5 months of age. a Representative confocal imaging of an amyloid plaque from the 4.5-month-old groups with IBA1 in green, X04 in blue, TR in red and the merge of X04/TR in pink. n = 6 mice per group (3 male and 3 female). APP/E3 n = 57, APP/E3/Trem2kon = 54, APP/E4 n = 130, APP/E4/Trem2kon = 94 individual plaques. b Quantification of the growth volume in 30 days for individual plaques. c The exposed area for each plaque is determined by the area in which microglia processes are not contacting the surface of TR (purple color in panel e). d Correlation between plaque growth and exposed surface area for each plaque and genotype. e Imaris generated 3D volumetric representations of an amyloid plaque from the 4.5-month-old group with IBA1 in green, exposed area in purple, TR in red, and surface contact in yellow. Analysis by one-way ANOVA followed by Tukey’s multiple comparison test. Bars represent mean ± SEM. * p < 0.05; *** p < 0.001